Visualizing intracellular nanostructures of living cells
using nanoendoscopy-AFM

7th January 2022by admin0

Atomic force microscopy (AFM) offers a method for
label-free imaging of nanoscale biomolecular dynamics to solve
biological questions that cannot be addressed via other bioimaging
methods including fluorescence and scanning electron microscopy.
Since such imaging methods are only possible for biological systems
extracted from cells or reconstructed on solid substrates,
nanodynamics within living cells largely remain inaccessible with
existing bioimaging methods. In a new report now published in
Science Advances, Marcos Penedo and a research team in Nanolife
Science and biotechnology at the Kanazawa University in Japan,
overcame the limits of bioimaging by using nanoendoscopy-AFM.
During the process, they inserted a needle-like probe into a living
cell to present actin fiber, three-dimensional (3D) maps and 2D
nanodynamics of the inner scaffold of the membrane with
undetectable changes in cell viability. Unlike earlier AFM methods,
the nanoprobe directly accessed the target intracellular components
and explored the capabilities of AFM, including high-resolution
imaging, nanomechanical mapping and molecular recognition to expand
the observable range of intracellular structures in living
cells.

Leave a Reply

Your email address will not be published. Required fields are marked *

https://nfusion-tech.com/wp-content/uploads/2019/10/Logo_newfusion-footer.png
https://nfusion-tech.com/wp-content/uploads/2019/10/Logo_newfusion-footer.png
Subscribe

If you wish to receive our latest news in your email box, just subscribe to our newsletter. We won’t spam you, we promise!

    New Fusion

    The New Fusion technology is based on a phenomenon called triplet-triplet annihilation (TTA) which is a process in which two triplet excitons annihilate and produce a higher energy singlet exciton.

    Subscribe

    If you wish to receive our latest news in your email box, just subscribe to our newsletter. We won’t spam you, we promise!

      New Fusion

      The New Fusion technology is based on a phenomenon called triplet-triplet annihilation (TTA) which is a process in which two triplet excitons annihilate and produce a higher energy singlet exciton.

      Copyright ©2024 New Fusion All Rights Reserved

      Designed by FallingBrick